This is a PhD project, with the aim to design a diagnostic biosensor for the rapid detection of bacteria. The current focus is on the identification of Clostridium difficile, a Gram-positive bacterium that can cause Clostridium difficile infection (CDI). CDI is the major cause of nosocomial antibiotic-associated diarrhoea. The symptoms of CDI range from, mild diarrhoea to pseudomembranous colitis (PMC), the latter having a mortality rate of 6-30%. Current diagnostic techniques for CDI are either time-consuming or have low-sensitivity. The aim of this research to design a rapid and accurate diagnostic system, for CDI. The project is multidisciplinary, involving bioinformatics, biosensor development and bacteriology.